- 作者: Paola Cuccarolo; Francesca Barbieri; Monica Sancandi; Silvia Viaggi; Paolo Degan
- 作者服務機構: Department of Epidemiology, Prevention and Special Functions, National Institute for Cancer Research (IST), Genova, Italy
- 中文摘要: --
- 英文摘要:
Background: Whether microgravity might influence tumour growth and carcinogenesis is still an open issue. It
is not clear also if and how normal and transformed cells are differently solicited by microgravity.
The present study was designed to verify this issue.
Methods: Two normal, LB and HSC93, and two transformed, Jurkat and 1310, lymphoblast cell lines were
used as representative for the two conditions. Two lymphoblast lines from Fanconi's anemia
patients group A and C (FA-A and FA-C, respectively), along with their isogenic corrected
counterparts (FA-A-cor and FA-C-cor) were also used. Cell lines were evaluated for their
proliferative ability, vitality and apoptotic susceptibility upon microgravity exposure in
comparison with unexposed cells. Different parameters correlated to energy metabolism, glucose
consumption, mitochondrial membrane potential (MMP), intracellular ATP content, red-ox
balance and ability of the cells to repair the DNA damage product 8-OHdG induced by the
treatment of the cells with 20mM KBrO3 were also evaluated.
Results: Transformed Jurkat and 1310 cells appear resistant to the microgravitational challenge. On the
contrary normal LB and HSC93 cells display increased apoptotic susceptibility, shortage of energy
storages and reduced ability to cope with oxidative stress. FA-A and FA-C cells appear resistant to
microgravity exposure, analogously to transformed cells. FA corrected cells did shown
intermediate sensitivity to microgravity exposure suggesting that genetic correction does not
completely reverts cellular phenotype.
Conclusions: In the light of the reported results microgravity should be regarded as an harmful condition either
when considering normal as well as transformed cells. Modeled microgravity and space-based
technology are interesting tools in the biomedicine laboratory and offer an original, useful and
unique approach in the study of cellular biochemistry and in the regulation of metabolic pathways. - 中文關鍵字: --
- 英文關鍵字: --