- 作者: 楊盛行; 高健元
- 作者服務機構: 國立臺灣大學農業化學系
- 中文摘要: Streptomyces rimosus以3%EMS處理,由5457個菌落於致死率6.6-66.7%下篩選得29個營養需求變異株,分別為AM-1~AM-29。由不同營養需求之三組變異株,AM-3和AM-27,,AM-7和AM-29,和AM-3和AM-21於50%PEG 1000在25℃進行原生質體融合30分鐘,而獲得25個融合株,分別為f.1~f-25。在固態醱酵時,20%融合株其地靈黴素產量比母株高;而在液態醱酵,則有44%融合株較母株高。融合株f-1,f-6,f-11,f-12,f-20和f-21在液態培養時地靈黴素產量較高而在固態培養時則反是;而融合株f-8則在固態醱酵時較高而液態培養時則反是。可見地靈黴素的生產之較佳菌株視固態或液態醱酵而定。
- 英文摘要: Streptomyces rimosus TM-55 was treated with 3% EMS, and 29 auxotrophic mutants(AM-1-AM-29) were isolated from 5457 colonies at a survival rate between 6.6-66.7%.Three sets of the auxotrophic mutant, AM-3 and AM-27, AM-7 and AM-28,and AM-3 andAM-21, were chosen for protoplast fusion with 50% PEG 1000 for 30 minutes at 25℃, and25 fusants were isolated (f.1-f-25). In solid substrate, oxytetracycline production of 20%fusants was higher than that of the wild strain, while in submerged fermentation, it was 44%.Oxytetracycline productions of fusants f-1. f-6, f-11, f-12, f-20 and f-21 were lower than thatof the wild strain in solid substrate, but this was reversed in submerged fermentation. On theother hand, OTC production of fusant f-8 was higher than that of the wild strain in solidsubstrate, and this was reversed in submerged fermentation.
- 中文關鍵字: Streptomyces rimosus; oxytetracycline production; protoplast fusion; solid state and submerged fermentation
- 英文關鍵字: --