- 作者: Nanna S. Mikkelsen & Rasmus O. Bak
- 作者服務機構: Department of Biomedicine, Aarhus University, Høegh-Guldbergsgade 10, Bldg. 1115, 8000, Aarhus C., Denmark
- 中文摘要:
- 英文摘要:
Genome editing technologies hold great promise for numerous applications including the understanding of cellular
and disease mechanisms and the development of gene and cellular therapies. Achieving high editing frequencies is
critical to these research areas and to achieve the overall goal of being able to manipulate any target with any desired
genetic outcome. However, gene editing technologies sometimes sufer from low editing efciencies due to several
challenges. This is often the case for emerging gene editing technologies, which require assistance for translation into
broader applications. Enrichment strategies can support this goal by selecting gene edited cells from non-edited cells.
In this review, we elucidate the diferent enrichment strategies, their many applications in non-clinical and clinical settings, and the remaining need for novel strategies to further improve genome research and gene and cellular therapy
studies. - 中文關鍵字:
- 英文關鍵字: s CRISPR/Cas, Enrichment, Selection, Reporter, Integration, INDELs, HDR, NHEJ, FACS, MACS, Surrogate reporter, Genetically modifed cells, Genome editing, Co-editing