- 作者: Lukkana Suksanpaisan, Tharinee Susantad, Duncan R. Smith
- 作者服務機構: Molecular Pathology Laboratory, Institute of Molecular Biology and Genetics, Mahidol University, Salaya campus, Nakorn Pathom, Thailand
- 中文摘要: --
- 英文摘要:
Background:
Despite infections by the dengue virus being a significant problem in tropical and subtropical
countries, the mechanism by which the dengue virus enters into mammalian
cells remains poorly described.
Methods:
A combination of biochemical inhibition, dominant negative transfection of Eps15
and siRNA mediated gene silencing was used to explore the entry mechanism of
dengue into HepG2 cells.
Results:
Results were consistent with entry via multiple pathways, specifically via clathrin
coated pit mediated endocytosis and macropinocytosis, with clathrin mediated
endocytosis being the predominant pathway.
Conclusions:
We propose that entry of the dengue virus to mammalian cells can occur by multiple
pathways, and this opens the possibility of the virus being directed to multiple cellular
compartments. This would have significant implications in understanding the
interaction of the dengue virus with the host cell machinery.
- 中文關鍵字: --
- 英文關鍵字: --