- 作者: Bishnuhari Paudyal, Pramila Paudyal, Dilip Shah, Hideyuki Tominaga, Yoshito Tsushima, Keigo Endo
- 作者服務機構: Department of Radiology, Thomas Jefferson University, Philadelphia, PA , USA
- 中文摘要: --
- 英文摘要:
Background:
The aim of this study was to develop the near infrared fluorescence (NIRF)-based imaging agent for the visualization of vascular endothelial growth factor (VEGF) in colon cancer. AlexaFluor 750 conjugating with bevacizumab, and injected intravenously into nude mice bearing VEGF over-expressing HT29 human colorectal cancer. Optical imaging was performed at 15 min, 24 h and 48 h post injection. Immunofluorescences staining of the tumor sections were performed. HT29 colorectal cancer xenografts were clearly visualized with bevacizumab-AlexaFluor 750.
Results:
Ex vivo analysis showed 2.1 ± 0.4%, 37.6 ± 6.3% and 38.5 ± 6.2% injected dose/g accumulated in the tumors at 15 min, 24 h and 48 h respectively. Tumor uptake was significantly decreased in pretreated with excess of bevacizumab (p = 0.002). Immunofluorescence analysis showed strong staining of anti-CD 31 antibody around the blood vessels. Anti-VEGF-A and bevacizumab showed heterogeneous expression throughout the tumor.
Conclusions:
Current study successfully detected the VEGF expression in HT29 colorectal cancer xenografts, signifying as a potential agent for non-invasive imaging of VEGF expression, which may be applied in clinical practice. - 中文關鍵字: --
- 英文關鍵字: Near infrared fluorescence; VEGF; Optical Imaging; Bevacizumab; Colorectal cancer