- 作者: Huey-Chung Huang; Po-Yuan Chang; Karen Chang; Chao-Yu Chen; Chung-Wu Lin; Jyh-Horng Chen; Chung-Yuan Mou; Zee-Fen Chang; Fu-Hsiung Chang
- 作者服務機構: Institute of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University, Taipei, Taiwan, R.O.C.
- 中文摘要: --
- 英文摘要:
Background :
Application of superparamagnetic iron oxide nanoparticles (SPIOs) as the contrast agent
has improved the quality of magnetic resonance (MR) imaging. Low efficiency of
loading the commercially available iron oxide nanoparticles into cells and the cytotoxicity
of previously formulated complexes limit their usage as the image probe. Here, we
formulated new cationic lipid nanoparticles containing SPIOs feasible for in vivo
imaging.
Methods :
Hydrophobic SPIOs were incorporated into cationic lipid 1,2-dioleoyl-3-
(trimethylammonium) propane (DOTAP) and polyethylene-glycol-2000-1,2-distearyl-3-
sn-phosphatidylethanolamine (PEG-DSPE) based micelles by self-assembly procedure to
form lipid-coated SPIOs (L-SPIOs). Trace amount of Rhodamine-dioleoylphosphatidylethanolamine
(Rhodamine-DOPE) was added as a fluorescent indicator.
Particle size and zeta potential of L-SPIOs were determined by Dynamic Light Scattering
(DLS) and Laser Doppler Velocimetry (LDV), respectively. HeLa, PC-3 and Neuro-2a
cells were tested for loading efficiency and cytotoxicity of L-SPIOs using fluorescent
microscopy, Prussian blue staining and flow cytometry. L-SPIO-loaded CT-26 cells were
tested for in vivo MR imaging.
Results :
The novel formulation generates L-SPIOs particle with the average size of 46 nm. We
showed efficient cellular uptake of these L-SPIOs with cationic surface charge into HeLa,
PC-3 and Neuro-2a cells. The L-SPIO-loaded cells exhibited similar growth potential as
compared to unloaded cells, and could be sorted by a magnet stand over ten-day duration.
Furthermore, when SPIO-loaded CT-26 tumor cells were injected into Balb/c mice, thegrowth status of these tumor cells could be monitored using optical and MR images.
Conclusions :
We have developed a novel cationic lipid-based nanoparticle of SPIOs with high loading
efficiency, low cytotoxicity and long-term imaging signals. The results suggested these
newly formulated non-toxic lipid-coated magnetic nanoparticles as a versatile image
probe for cell tracking. - 中文關鍵字: --
- 英文關鍵字: --