- 作者: Aseervatham Anusha Amali; Ravikumar Deepa Rekha; Cliff Ji-Fan Lin; Wei-Lun Wang; Hong-Yi Gong; Gour-Mour Her; Jen-Leih Wu
- 作者服務機構: 1 Laboratory of Marine Molecular Biology and Biotechnology 301, Institute Cellular and Organismic Biology, Academia S璯ica, 128, Academia Road, Section 2, NanKang, Taipei, 11529, Taiwan; ; 2 Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan; ; 3 Institute of Fisheries Sciences, National Taiwan University, Taipei, Taiwan;; 4 Graduate Institute of Biotechnology, National Taiwan Ocean University, Keelung, Taiwan
- 中文摘要: --
- 英文摘要: Steatohepatitis has recently been increasing as a cofactor influencing the progression of fibrosis, cirrhosis, adenoma and carcinoma in liver; however, the mechanisms by which it contributes to liver injury remain uncertain. We induced steatohepatitis in zebrafish embryos using thioacetamide (TAA). TUNEL assay revealed significant increasing of apoptosis in liver after 5 days post fertilization and the increasing of apoptosis was observed to be associated with the up-regulation of apoptotic genes such as, bad, bax, P-38a, caspase-3 and 8, and JNK-1. Histological sections by oil red O stain showed the accumulation of fatty droplets which causes the pushing of the nucleus towards one side. Up-regulation of steatosis markers such as, ACC, adiponectin, PTL, CEBP- α and β, SREBP-1 was also observed. Furthermore, the elevation of glutathione peroxidase in TAA treated embryos indicated that TAA induces lipid peroxidation which leads to causes liver damage. Zebrafish has already been considered as a good human disease model and in this context; TAA-treated zebrafish may serve as a good animal model to study the molecular pathogenesis of steatohepatitis. Moreover, non-availability of specific drugs to prevent steatohepatitis, this animal model may serve as a powerful preclinical platform to study the therapeutic strategies and for evaluating che-moprevention strategies for this disease.
- 中文關鍵字: --
- 英文關鍵字: liver, steatosis, thioacetamide, zebrafish