- 作者: Yeong-Shiau Pu, Chao-Yuan Huang, Yi-Zih Kuo, Wang-Yi Kang, Guang-Yaw Liu, A-Mei Huang, Hong-Jeng Yu, Ming-Kuen Lai, Shu-Pin Huang, Wen-Jeng Wu, Shean-Jaw Chiou, Tzyh-Chyuan Hour
- 作者服務機構: Department of Urology, National Taiwan University College of Medicine, Taipei, Taiwan, R.O.C.
- 中文摘要: --
- 英文摘要:
Metastatic renal cell carcinoma (RCC) is highly resistant to conventional
systemic treatments, including chemotherapy, radiotherapy and hormonal
therapies. Previous studies have shown over-expression of EGFR is
associated with high grade tumors and a worse prognosis. Recent studies
suggest anticancer therapies targeting the EGFR pathway have shown
promising results in clinical trials of RCC patients. Therefore, characterization
of the level and localization of EGFR expression in RCC is important for
target-dependent therapy. In this study, we investigated the clinical
significance of cellular localization of EGFR in human normal renal cortex
and RCC. RCC and adjacent normal kidney tissues of 63 patients were
obtained for characterization of EGFR expression. EGFR protein expression
was assessed by immunohistochemistry on a scale from 0 to 300
(percentage of positive cells staining intensity) and Western blotting.
EGFR membranous staining was significantly stronger in RCC tumors than
in normal tissues (P<0.001). In contrast, EGFR cytoplasmic staining was
significantly higher in normal than in tumor tissues (P<0.001). The levels of
membranous or cytoplasmic EGFR expression in RCC tissues were not
correlated with sex, tumor grade, TNM stage or overall survival (P>0.05).
These results showed abundant expression of membranous EGFR in RCC,
and abundant expression of cytoplasmic EGFR in normal tissues. EGFR
expression in RCC was mostly located in the cell membrane, whereas the
EGFR expression in normal renal tissues was chiefly seen in cytoplasm. Our
results suggest different locations of EGFR expression may be associated
with human renal tumorigenesis. - 中文關鍵字: --
- 英文關鍵字: --