• 作者： Hsu, Jyh-Ping; Tsao, Heng-Kwong
• 中文摘要： 我們假設在固體表面上鄰近的動物細胞可以形成一個微環境。由於在此微環境中的自催化生長因子之濃度可達一生長所需的臨界值,在其中的細胞可進行分裂增殖。如果接種至固體表面的細胞無法形成微環境,則它們無法生長。基於這個假設,許多在動物細胞培養中觀察到的奇特現象都可以合理的被解釋。本文解答了以下幾個問題:(1)為何存在一臨界接種密度?(2)當細胞生長在微載體之表面時,臨界接種細胞數為何不與微載體之表面積成正比?(3)為何即使在極低的接種密度下,有些微載體表面仍然可以觀察到細胞的成長?(4)為何當細胞在培養皿上成長時對接種密度之需求遠低於在微載體上成長時對接種密度之需求?(5)就細胞在培養皿上之成長而言,為何接種效率會隨著接種密度改變?如何改變?。
• 英文摘要： We assume that adjacent cells on a solid surface may establish a microenvironment with a concentration of an autocrine growth factor (AGF) greater than a threshold value. Cells enclosed in this microenvironment undergo proliferation as a response to the regulatory action of AGF. If no such microenvironment can be formed, cells remain unchanged on the surface. On the basis of this hypothesis, all the seemingly peculiar behaviors of the cell growth phenomenon under consideration become logical. In particular, the following problems are solved: (i) Why does there exist a critical inoculum density? (ii) Why is the critical inoculum cell number proportional to the radius of a microcarriers with a power less than two? (iii) Why is cell growth on microcarriers appreciable even at a very low level of inoculum density? (iv) Why is the inoculum density requirement for cells growing on petri dishes considerably less than that for cells growing on microcarriers? (v) Why does plating efficiency vary with inoculum density for cells growing on petri dishes, and how does it vary?
• 中文關鍵字： 動物細胞生長; 勾引性; 自催化生長因子; 蒙地卡羅模擬
• 英文關鍵字： Mammalian Cell Growth; Anchorage Dependent; Autocrine Growth Factor; Monte Carlo Simulation