- 作者: Ben-Tzu Chiang; Yi-Wen Liu; Ben-Kuen Chen; Ju-Ming Wang; Wen-Chang Chang
- 作者服務機構: 1 Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan, 701, Taiwan; ; 2 Center for Gene Regulation and Signal Transduction Research, National Cheng Kung University, Tainan, Taiwan; ; 3 Graduate Institute of Biopharmaceutics, College of Life Sciences, National Chiayi University, Chiayi, Taiwan
- 中文摘要: --
- 英文摘要: We previously reported that LPS activates the transcription of the IL-10 gene through the Sp1 and C/EBP binding sites and indicated that Sp1, C/EBPβ and C/EBPδ can coactivate the IL-10 gene expression in mouse macrophage cells [Liu Y.-W., Tseng H.-P., Chen L.-C, Chen B.-K., Chang W.-C. J. Immunol. 171: 821-828, 2003]. In the present report, we demonstrated the direct physical interaction between C/EBPδ and Sp1, and also mapped the interaction domains of these two proteins. C/EBPδ binds to Sp1 via its basic region leucine zipper domain. The C-terminus of Sp1 was also the major region interacting with C/EBPδ. However, both glutamine- and serine/threonine-rich homologus regions of Sp1 also interacted with C/ EBPδ. The binding of Sp1 and C/EBPδ as a complex to the Sp1 binding site on the promoter of IL-10 was further confirmed by using the DNA affinity precipitation assay. By using Sp1-deficient SL2 cells, we also found that the overexpressions of C/EBPδ and Sp1 synergically activate the transcriptional activity of IL-10 gene. Taken together, our present results revealed a novel mechanism of a superactivation of Spl by C/ EBPδ via a direct interaction between these two transcription factors leading to the activation of the IL-10 gene in mouse macrophage cells.
- 中文關鍵字: --
- 英文關鍵字: cytokines, gene regulation, monocytes/macrophages, signal transduction, transcription factors