- 作者: Anna M Czarnecka; Jerzy S Czarnecki; Wojciech Kukwa; Francesco Cappello; Anna Scinska; Andrzej Kukwa
- 作者服務機構: Laboratory of Molecular Oncology, Department of Oncology, Military Institute of the Health Services, Warsaw, Poland
- 中文摘要: --
- 英文摘要:
Mitochondria are sub-cellular organelles that produce adenosine triphosphate (ATP)
through oxidative phosphorylation (OXPHOS). As suggested over 70 years ago by Otto
Warburg and recently confirmed with molecular techniques, alterations in respiratory
activity and in mitochondrial DNA (mtDNA) appear to be common features of malignant
cells. Somatic mtDNA mutations have been reported in many types of cancer cells, and
some reports document the prevalence of inherited mitochondrial DNA polymorphisms
in cancer patients. Nevertheless, a careful reanalysis of methodological criteria and
methodology applied in those reports has shown that numerous papers can’t be used as
relevant sources of data for systematic review, meta-analysis, or finally for establishment
of clinically applicable markers.
In this review technical and conceptual errors commonly occurring in the
literature are summarized. In the first place we discuss, why many of the published
papers cannot be used as a valid and clinically useful sources of evidence in the
biomedical and healthcare contexts. The reasons for introduction of noise in data and in
consequence - bias for the interpretation of the role of mitochondrial DNA in the complex
process of tumorigenesis are listed. In the second part of the text practical aspects of
mtDNA research and requirements necessary to fulfill in order to use mtDNA analysis in
clinics are shown. Stringent methodological criteria of a case-controlled experiment in
molecular medicine are indicated. In the third part we suggest, what lessons can be
learned for the future and propose guidelines for mtDNA analysis in oncology. Finally
we conclude that, although several conceptual and methodological difficulties hinder the research on mitochondrial patho-physiology in cancer cells, this area of molecular
medicine should be considered of high importance for future clinical practice. - 中文關鍵字: --
- 英文關鍵字: --