- 作者: Bin Zeng; Guosheng Lin; Xiaofeng Ren; Yan Zhang; Honglei Chen
- 作者服務機構: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei, China
- 中文摘要: --
- 英文摘要:
Heme oxygenase-1 (HO-1) is a stress-inducible enzyme with diverse cytoprotective
effects, and reported to have an important role in angiogenesis recently. Here we
investigated whether HO-1 transduced by mesenchymal stem cells (MSCs) can induce
angiogenic effects in infarcted myocardium. HO-1 was transfected into cultured
MSCs using an adenoviral vector. 1×106 Ad-HO-1-transfected MSCs (HO-1-MSCs)
or Ad-Null-transfected MSCs (Null-MSCs) or PBS was respectively injected into rat
hearts intramyocardially at 1 h post-myocardial infarction. The results showed that
HO-1-MSCs were able to induce stable expression of HO-1 in vitro and in vivo. The
capillary density and expression of angiogenic growth factors, VEGF and FGF2 were
significantly enhanced in HO-1-MSCs-treated hearts compared with
Null-MSCs-treated and PBS-treated hearts. However, the angiogenic effects of HO-1
were abolished by treating the animals with HO inhibitor, zinc protoporphyrin. The
myocardial apoptosis was marked reduced with significantly reduced fibrotic area in
HO-1-MSCs-treated hearts; Furthermore, the cardiac function and remodeling were
also significantly improved in HO-1-MSCs-treated hearts. Our current findings
support the premise that HO-1 transduced by MSCs can induce angiogenic effects and
improve heart function after acute myocardial infarction. - 中文關鍵字: --
- 英文關鍵字: --