- 作者： 劉文雄; 許榮欣; 王煒
- 作者服務機構： 國立臺灣大學農業化學系
- 中文摘要：
以紫外線照射法對從臺灣土壤中新分離之誘導性膽固醇氧化?生產菌株Arthrobacter simplex B-7進行人工誘導變
異，並從分離之各種變異株中篩選出一株Streptomycin 及 Ampicillin耐性之優良誘導性酵素生產變異株U-S-A-18及一株
構成性酵素生產變異株U-0-24。在最適當酵素生產條件下變異株U-S-A-18及U-0-24 之膽固醇氧化?生產量分別為母株
之9及2.3倍，顯示此二變異株均為具有工業生產潛力之膽固醇氣化?生產菌株。
0 - 英文摘要： An inducible cholesterol oxidase-producing bacterium, Arhtrobacter simplex B-7, which was newlyisolated from Taiwan soil was improved by an ultraviolet radiation mutation. Among various mutantsisolated, mutants U-S-A-18 and U-O-24 were selected for the high potency in the production of cholesteroloxidase. Mutant U-S-A-18, a streptomycin and ampicillin resistant mutant, is an inducible enzyme pro-ducer；mutant U-O-24 is a constitutive enzyme producer. Under optimum fermentation conditions, thecholesterol oxidase productivities of mutant U-S-A-18 and mutant U-O-24 were 9- and 2.3-fold those ofthe parent strain, respectively. It indicates that both the inducible mutant U-S-A-18 and the constitutivemutant U-O-24 are cholesterol oxidase-producing microorganisms of industrial potentiality.
- 中文關鍵字： --
- 英文關鍵字： --
