第7卷‧第4期,
198310
, pp. 481-487
人體胎盤甲硫胺酸活化?對動力學機轉
- 作者：
周慰遠; 潘福
- 作者服務機構：
國防醫學院生物化學研究所
- 中文摘要：
由前文之胺酸活化反應及本文之核酸酯化反應研究結果綜合研判，人體甲硫胺酸活化酯之動力學機轉與鼠肝甲硫
胺酸活化?不同，而與人體丁羥胺酸活化?類似，屬於基質結合不定序而生成物釋出順序之二一一二交替型，以單磷
酸腺?為最後釋出之生成物，單磷酸腺?及焦磷酸兩種反應生成物均可能與?及甲硫胺酸複合物結合形成無反應複合
物。
- 英文摘要：
The order of substrate binding and product release of human placental methionyl-tRNA synthetasewas investigated in the aminoacylation reaction by the initial velocity and product inhibition experiments.The kinetic patterns obtained from the present studies and those from our previous studies in the ATP-PP exchange reaction are consistent with a Bi(random) Uni Uni Bi (ordered) ping-pong mechanism, with dead-end complex formation both by PP and AMP, the first and last product, respectively. The proposed kineticmechanism is similar to that for human placental threonyl-tRNA synthetase (Pan, et al., Int. J. Peptideprotein Res., 20, 159, 1982) rather than that for rat liver methionyl-tRNA synthetase (Pan, et al., J. Chin.Biochem. Soc., 4, 17, 1975).
- 中文關鍵字：
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- 英文關鍵字：
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