- 作者: 趙壯飛; 鍾明芳; 陳正繹; 曾月貌; 許玉珠
- 作者服務機構: 國防醫學院生物及解剖學科
- 中文摘要:
在動物及人類紅血球中可發現17-B-氫氧基類固醇去氫?之存在,有些作者認為存在之意義可能為負擔調節血液中雌
素二醇之濃度或血球中雌素二醇之含量。但仍無具體證據支持。而紅血球是由骨髓中紅血球母細胞經一系列增生分化後
之最終分化細胞,因此作者相信紅血球中所含之17-B-氫氧基類固醇去氫?為骨髓紅血球母細胞之殘留物。甚多實驗證
實,雌素二醇具有抑制骨髓紅血球生長分化作用。因此17-B-氫氧基類固醇去氫?存在於骨髓紅血球母細胞中,可能具
有調節細胞中雌素二醇之生化作用。本實驗乃利用放血或注射phenyl-hydrazine以誘導大白兔骨髓紅血球系列母細胞增生
,再以細胞化學及生化方法觀察分析17-B-氫氧基類固醇去氫?之活性改變。結果顯示經由放血或phenyl-hydrazine處
理大白兔,其骨髓中紅血球系列細胞增加約二至三倍,同時17-B-氫氧基類固醇去氫活性亦增加二至三倍,因此相信17-
B-氫氧基類固醇去氫?可調節紅血球母細胞中雌素二醇之含量,進而調節紅血球細胞之增生及分化作用。 - 英文摘要: Because of the general presence of 17-B-hydroxysteroid dehydrogenase (17-B-HSD) in animals andhuman erythrocytes, it is very likely that this enzyme is also present in the erythrocyte precursor cells inbone marrow. Adult male white New Zealand rabbits were used to demonstrate the existence of the 17-B-HSD in bone marrow cells and to evaluate the biological function of 17-B-HSD in the erythropoieticsystem in bone marrow. Anemia was artificially induced in these animals in order to stimulate the pro-liferation of erythropoietic cells. This was accomplished by bleeding or by intra-peritoneal injection of40 mg/kg of phenylhydrazine. The 17-B-HSD activities were then examined by histochemical and bio-chemical assays. The results showed a two to three-fold increase of the enzyme activities in both peripheralerythrocytes and bone marrow cells of anemic animals. Our experiments further demonstrate the fine con-trol of erythropoiesis. The enzyme, which favors the conversion of oestradiol to oestrone, is itself boostedby an anemic state. As a result, the inhibitory action of hemopoiesis by oestradiol was relieved.
- 中文關鍵字: 17-B-HSD; erythropoietic stem cell; anemia-induced rabbit
- 英文關鍵字: --