- 作者: 黃得時 ; 曾怡禛 ; 劉惠雲 等
- 作者服務機構: 成功大學化學系
- 中文摘要:
Acute toxicity of cresols to both Pseudomonas I and II was estimated by an initial oxygen uptake method. Inhibition studies of toluene and cresols on the oxidation of either benzoate by Pseudomonas I or phenol by Pseudomonas II were analyzed and expressed as oxygen uptake rates. Double reciprocal plots for the inhibition by cresols of oxygen uptake in Pseudomonas, two physical constants, Vmaxi and Ki, were obtained. The Vmaxi of o-, m- and p-cresol were 80%, 81% and 57% of Vmax in Pseudomonas I, and 10%, 25% and 36% in Pseudomonas II, respectively. Thus, the toxicity to Pseudomonas I decreases in the order p-> o-.gtoreq.m-cresol, whereas to Pseudomonas II, the order is changed to o->m->p-cresol. This difference in the toxicity order is probably due to the allosteric effect of p-cresol towards Pseudomonas II.
Inasmuch as most compounds inhibit noncompetively, the relative toxicity of different compounds can be estimated by a new toxicity parameter RI (relative inhibition) which is defined as 100/Ki. By comparing the RI value of each compound, the toxicity to Pseudomonas I decreases in the order m-chlorophenol>p-cresol>p-chlorophenol> o-cresol.gtoreq.m-cresol>o-chlorophenol>toluene>phenol. - 英文摘要: --
- 中文關鍵字: Cresol; Pseudomonas; Methyl Phenol; Chlorophenol; Toxicity; Inhibition
- 英文關鍵字: 甲酚;假單孢菌屬;甲基酚;氯酚;毒性;抑制