- 作者: Ho-Sheng Wu; Yueh-Chun Hsieh; Ih-Jen Su; Ting-Hsiang Lin; Shu-Chun Chiu; Yu-Fen Hsu; Jih-Hui Lin; Mei-Ching Wang; Jeou-Yuan Chen; Pei-Wen Hsiao; Geen-Dong Chang; Andrew H.-J. Wang; Hsieh-Wei Ting; Chih-Ming Chou; Chang-Jen Huang
- 作者服務機構: Graduate Institute of Life Sciences, National Defense Medical Center, Division of Laboratory Researc and Development, Center for Disease Control, Department of Health, Institutes of Biological Chemistry, Biomedical Sciences, and BioAgricultural Sciences, Academia Sinica, Graduate Institute of Biochemical Sciences, National Taiwan University, and Department of Biochemistry, Taipei Medical University, Taipei, Taiwan, ROC
- 中文摘要: --
- 英文摘要: Severe acute respiratory syndrome(SARS),a new dis-ease with symptoms similar to those of atypical pneu-monia,raised a global alert in March 2003.Because of itsrelatively high transmissibility and mortality upon infec-tion,probable SARS patients were quarantined andtreated with special and intensive care. Therefore, in-stant and accurate laboratory confirmation of SARS-associated coronavirus(SARS-CoV)infection has be-come a worldwide interest. For this need, we purifiedrecombinant proteins including the nucleocapsid(N),en-velope(E),membrane(M),and truncated forms of thespike protein(S1-S7)of SARS-CoV in Escherichia coli.The six proteins N,E, M, S2, S5, and S6 were used forWestern blotting (WB)to detect various immunoglobulinclasses in 90 serum samples from 54 probable SARSpatients.The results indicated that N was recognized inmost of the sera. In some cases, S6 could be recognizedas early as 2 or 3 days after illness onset, whie S5 wasrecognized at a later stage. Furthermore, the result ofrecombinant-protein-based WB showed a 90% agree-ment with that of the whole-virus-based immunofluores-cence assay. Combining WB with existing RT-PCR, thelaboratory confirmation for SARS-CoV infection wasgreatly enhanced by 24.1%,from 48,1%(RT-PCR alone)to 72.2%.Finally, our results show that IgA antibodiesagainst SARS-CoV can be detected within 1 week afterillness onset in a few SARS patients.
- 中文關鍵字: --
- 英文關鍵字: --