- 作者: Miroslav Varecha; Michal Zimmermann; Jana Amrichova; Vladimir Ulman; Pavel Matula; Michal Kozubek
- 作者服務機構: Centre for Biomedical Image Analysis, Faculty of Informatics, Masaryk University, Czech Republic
- 中文摘要: --
- 英文摘要:
During apoptosis several mitochondrial proteins are released. Some of them participate in caspase-
independent nuclear DNA degradation, especially apoptosis-inducing factor (AIF) and
endonuclease G (endoG). Another interesting protein, which was expected to act similarly as AIF
due to the high sequence homology with AIF is AIF-homologous mitochondrion-associated inducer
of death (AMID). We studied the structure, cellular localization, and interactions of several proteins
in silico and also in cells using fluorescent microscopy. We found the AMID protein to be
cytoplasmic, most probably incorporated into the cytoplasmic side of the lipid membranes.
Bioinformatic predictions were conducted to analyze the interactions of the studied proteins with
each other and with other possible partners. We conducted molecular modeling of proteins with
unknown 3D structures. These models were then refined by MolProbity server and employed in
molecular docking simulations of interactions. Our results show data acquired using a combination
of modern in silico methods and image analysis to understand the localization, interactions and
functions of proteins AMID, AIF, endonuclease G, and other apoptosis-related proteins. - 中文關鍵字: --
- 英文關鍵字: --