- 作者: Xiao Jian; Zhu Xiao-yan; He Bin; Zhang Yufeng; Kang Bo; Wang Zhi-nong; Ni Xin
- 作者服務機構: Department of cardiothoracic surgery, Changzheng hospital, the second millitary medical university, shanghai, Mainland China
- 中文摘要: --
- 英文摘要:
Background: Autophagy plays a significant role in myocardial ischemia-reperfusion (IR) injury. So it is important to inhibit autophagy to protect cardiomyocytes besides anti-apoptosis. MiRNA has been demonstrated to protect cardiomyocytes against apoptosis during IR, while whether it has anti-autophagy effect has not been known. The aim of this study was to investigate whether miR-204 regulated autophagy by regulating LC3-II protein, which is the marker of autophagosome during myocardial IR injury.
Methods: Adult SD rats were randomized to Control and IR groups. IR group was treated with 30min ischemia by ligating the left anterior descending coronary artery, followed by 2h reperfusion by loosing the ligation. The expression of miR-204 was measured by RT-PCR, and LC3 protein was measured by western-blot.
Results: We found that IR induced cardiomyocytes autophagy, together with down-regulation of miR-204 and up-regulation of LC3-II protein. And, we have found that LC3-II protein was regulated by miR-204, using the method of transferring miR-204 mimic or AMO-204 into the cardiomyocytes, before.
Conclusions: These studies provided evidence that miR-204 played an important role in regulating autophagy through LC3-II protein during IR.
- 中文關鍵字: --
- 英文關鍵字: --