- 作者: T Peeyush Kumar; Sherin Antony; G Gireesh; Naijil George; C s Paulose
- 作者服務機構: Molecular Neurobiology and Cell Biology Unit, Centre for Neuroscience, Cochin University of Science and Technology, Kerala, India
- 中文摘要: --
- 英文摘要:
Curcumin, an active principle component in rhizome of Curcuma longa, has
proved its merit for diabetes through its anti-oxidative and anti-inflammatory
properties. This study aims at evaluating the effect of curcumin in modulating the
altered dopaminergic receptors, CREB and phospholipase C in the cerebral cortex and
cerebellum of STZ induced diabetic rats. Radioreceptor binding assays and gene
expression was done in the cerebral cortex and cerebellum of male Wistar rats using
specific ligands and probes. Total dopaminergic receptor binding parameter, Bmax
showed an increase in cerebral cortex and decrease in the cerebellum of diabetic rats.
Gene expression studies using real time PCR showed an increased expression of
dopamine D1 and D2 receptor in the cerebral cortex of diabetic rats. In cerebellum
dopamine D1 receptor was down regulated and D2 receptor showed an up regulation.
Transcription factor CREB and phospholipase C showed a significant down
regulation in cerebral cortex and cerebellum of diabetic rats. We report that curcumin
supplementation reduces diabetes induced alteration of dopamine D1, D2 receptors,
transcription factor CREB and phospholipase C to near control. Our results indicate
that curcumin has a potential to regulate diabetes induced malfunctions of
dopaminergic signalling, CREB and Phospholipase C expression in cerebral cortex
and cerebellum and thereby improving the cognitive and emotional functions
associated with these regions. Furthermore, in line with these studies an interaction
between curcumin and dopaminergic receptors, CREB and phospholipase C is
suggested, which attenuates the cortical and cerebellar dysfunction in diabetes. These
results suggest that curcumin holds promise as an agent to prevent or treat CNS
complications in diabetes. - 中文關鍵字: --
- 英文關鍵字: --