• 作者： 姚鍾太1; 廖錦霞; 尹士俊
• 作者服務機構： 三軍總醫院外科部; 三軍總醫院醫學研究部; 國防醫學院生化學科
• 中文摘要： 醇脫氫?（ADH）和醛脫氫?（ALDH）是人體主要的酒精代謝?。此二?在不同種族表現遺傳多型性“我們研究23
  例肝手術檢體，探討酒精代謝?活性與遺傳多型性間的關係。應用瓊膠等電焦集電泳鑑定ADH與ALDH同功?表現
  型及紫外光可見光光譜儀動力測定?之活性。在pH 7.5，33 mM乙醇反應條件，同型接合子表現型ADH2 1-1和ADH2
  2-2及異型接合子表現型ADH2 1-2肝檢體?活性分別測得2.9 ± 0.7，16.0 ± 2.5及13.6 ± 1.0單位／克組織。在200 μM
  乙醛濃度，ALDH2活性型和ALDH2失活型肝檢體?活性分別測得1.06 ± 0.13及0.71 ± 0.07單位∕克組織。這項結
  果發現人類肝臟的酒精代謝?活性依ADH2和ALDH2基因位置多型性而顯著差異，並顯示束方人族群不同基因型肝之
  酒精代謝能力不同，會影響其飲酒行為與酒癮的發生。
  此
• 英文摘要： Alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) are the major enzymes re-sponsible for the metabolism of ethanol in the body. Both exhibit genetic polymorphism in racial popula-tions. To determine hepatic ethanol metabolizing activities in relation to genetic polymorphism, a total of 23surgical specimens were investigated. The expression patterns of ADH and ALDH isoenzymes were identi-fied by means of agarose isoelectric focusing, and the activities were assayed spectrophotometrically. At 33mM ethanol, pH 7.5, the activities in the liver with the homozygous phenotype ADH2 1-1 and ADH2 2-2and the heterozygous phenotype ADH2 1-2 were determined to be 2.9 ± 0.7, 16.0 ± 2.5, and 13.6 ± 1.0 U/gtissue, respectively. The activities of the ALDH2-active and ALDH2-inactive phenotypes at 200 M acetal-dehyde were determined to be 1.06 ± 0.13 and 0.71 ± 0.07 U/g tissue, respectively. These findings indicate thathuman hepatic ethanol-metabolizing activities differ significantly with respect to polymorphism at both theADH2 and ALDH2 loci. The results suggest that this genetically determined differential hepatic activitymay influence drinking behavior and the development of alcoholism among Orientals.
• 中文關鍵字： genetic polymorphism; liver; alcohol dehydrogenase; aldehyde dehydrogenase activity.
• 英文關鍵字： --