- 作者: 邱式鴻
- 作者服務機構: 台灣大學生化科學研究所
- 中文摘要: A rapid peptide-bond hydrolysis by means of microwave irradiation is introduced for the facile preparation of protein hydrolysates used for amino acid analysis. The optimal hydrolysis condition has been determined using several enzymes with known amino acid compositions. The effects of hydrolysis time on the recovery of various labile and hydrophobic amino acids are also exemplified in the microwave heating of standard amino acids. The method has been applied to the complete amino acid analysis with a single nonvolatile solvent of methanesulfonic acid with good recovery of tryptophan and half-cystine. It provides a radical expedition of protein and peptide hydrolysis via commercial microwave ovens and specially-designed Teflon- Pyrex tubes, circumventing the tedious procedures using vacuum-sealed pyrex tubes heating at 110.degree.C for more than 24h. This novel type of microwave chemistry associated with rapid peptide-bond cleavage is of great potential in the automation of the complete process of amino acid analysis starting from the preparation of protein hydrolysates.
- 英文摘要: --
- 中文關鍵字: Amino Acid Analysis; Microwave Irradiation; Rapid Protein Hydrolysis; Teflon-Pyrex Tubes
- 英文關鍵字: 胺基酸分析;微波放射;蛋白質迅速水解;耐熱試管