- 作者: 林仁混 ; 張素真 ; 黃□圻
- 作者服務機構: 台灣大學生化研究所
- 中文摘要:
The new chromophoric reagent, 4-dimethylaminoazobenzene-4'-sulfonyl chloride (dabsyl chloride) was synthesized by reaction of sodium 4-dimethylaminoazobenzene 4'-sulfonate with phosphorus pentachloride. Dabsyl chloride reacted rapidly with all amino acids, aliphatic amines, and polyamines to form the corresponding dabsyl derivatives. The dabsyl amino acids(10/sup -11/mol) were visualized on thin layer plates and found to be photo-stable. During the last 12 years, in combination with HPLC, this newly developed labeling reagent has been shown to be very promising for microdetermination of amino acids, aliphatic amines and polyamines.
Recently, another new chromophoric reagent, dabsylhydrazine, was synthesized from the reaction of dabsyl chloride with hydrazine. This reagent reacted readily with monosaccharides and carbonyl compounds to form the corresponding chromophoric dabsylhydrazones with strong absorbance at 425 nm. The application of this new reagent in HPLC analysis of monosaccharides was described. By use of Nova-PAK C/sub 18/ reverse-phase column and a concave gradient system of water and acetonitrile as eluent, the detection limits of monosaccharides in the range of 10 pmol have been reached. - 英文摘要: --
- 中文關鍵字: Amino Compounds; Carbonyl Compounds; Dabsyl Chloride; Dabsyl Hydrazine; High-Performance Liquid Chromatography
- 英文關鍵字: 氨基化合物;羰基化合物;4-二甲基氨基偶氮苯-4'磺醯氯;4-二甲基氨基偶氮苯-4'磺醯聯胺;高性能液相層析法