• 作者： 魏耀揮; 陳祐生; 李建富; 黃君瑜; 李家祥
• 作者服務機構： 國立陽明醫學院生化學科
• 中文摘要： 本研究以含各種不同乙醇濃度之等熱量液態飼料餵食大白鼠來探討飲用乙醇對肝粒線體功能的影響。我們發現在餵食此等液態飼料2到3個月之後，餵以含乙醇飼料的大白鼠之體重與對照組沒有顯著差異。然而，肝粒線體呼吸速率則隨著飼料中乙醇濃度的增加而有顯著的降低。我們發現餵食含10.8％乙醇飼料的大白鼠，其琥珀酸支持的 State3 平均呼吸速率從對照組的54.5降至44.8 nmol (方程式無法摘錄) ∕min∕mg，而由麩胺酸及蘋果酸支持的 State3呼吸速率則從對照組的38.8降至23.6 nmol (方程式無法摘錄) ∕min∕mg。很有趣地，我們注意到乙醇的攝取對State3呼吸的影響較其對State4呼吸的影響來得嚴重，而且這種現象與粒線體呼吸所利用的受質無關。另一方面，我們發現粒線體的呼吸控制商和ADP∕O比值也隨著大白鼠飼料中乙醇濃度的增加而同時降低。再者，乙醇對呼吸控制商和ADP∕O比值的影響，在麩胺酸及蘋果酸支持的呼吸上表現較琥珀酸支持的呼吸為顯著。 這些實驗結果很清楚地顯示，大白鼠攝取乙醇可導致肝粒線體呼吸與氧化磷酸化功能的損壞，而且這些作用是由於粒線體膜的破損所造成的。
• 英文摘要： The effect of ethanol intake on liver mitochondrial functions was investigated by feeding rats with aliquid isocaloric diet containing various concentrations of ethanol. We found that after feeding the liquiddiet for 2 to 3 months, the body weight of rats did not show a significant difference between treated andcontrol groups. However, the mitochondrial respiration rate decreased significantly with the increase ofethanol concentration in the diet. We found that when the rats were fed on 10.8% ethanol, the averagesuccinate-supported State 3 respiration rate decreased from 54.5 to 44.8 nmol /min/mg and the gluta-mate-malate-supported State 3 respiration rate decreased from 38.8 to 23.6 nmol /min/mg as comparedwith the control. Interestingly, we noted that ethanol intake caused a more drastic effect on State 3 re-spiration than on State 4 respiration, irrespective of the substrate utilized by the mitochondria. In addi-tion, the respiratory control and ADP/O ratios were found to decrease concomitantly with the increase ofethanol level in the diet. Moreover, we found that the effect of ethanol on both respiratory control andADP/O ratios of liver mitochondria was more pronounced in glutamate-malate-supported respiration thansuccinate-supported respiration. These results clearly demonstrate that ethanol intake by the rat can causeimpairment of liver mitochondrial respiration and oxidative phosphorylation，and that these effects areexerted through damage to mitochondrial membranes.
• 中文關鍵字： ethanol; mitochondria; respiratory control; ADP/O ratio
• 英文關鍵字： --