- 作者: Chi-Iou Lin; Chun-Hung Hsieh; Stanley Shiao-Ying Lee; Wei-Shan Lee; Gou-Ping Chang-Chien; Chien-Yuan Pan; Hsinyu Lee
- 作者服務機構: Institute of Zoology, National Taiwan University, Taipei, Taiwan, ROC
- 中文摘要: --
- 英文摘要:
Dioxins comprise a group of compounds which contain a double aromatic ring-like structure. They are
among the most prevalent and toxic environmental pollutants. Accumulation of dioxins in human tissues poses a
potential threat to human health. Currently, analytical chemical procedures dominate dioxin-detection protocols.
In this study, we established a fluorescence resonance energy transfer (FRET)-based dioxin-detection bioassay.
Aryl hydrocarbon receptor (AHR) and AHR nuclear translocator (ARNT) fused-cyan fluorescent protein (CFP)
and -yellow fluorescent protein (YFP) constructed were transiently co-transfected into rat hepatoma cell line,
H4IIEC3 cells. Our results showed that no FRET signals were detected in AHR-CFP- and ARNT-YFP-transfected
H4IIEC3 cells. However, dioxin treatments upregulated FRET signals in these transfected cells in a dose-dependent
manner. This work highlighted the potential of FRET technique in the detection of dioxin-like compounds. - 中文關鍵字: --
- 英文關鍵字: Dioxin; AHR; ARNT; Fluorescence resonance energy transfer