- 作者: Chung-Chun Wu, Chih-Yeu Fang, Yu-Jhen Cheng, Hui-Yu Hsu, Sheng-Ping Chou, Sheng-Yen Huang, Ching-Hwa Tsai and Jen-Yang Chen
- 作者服務機構: 1. National Institute of Cancer Research, National Health Research Institutes, No.35, Keyan Road, Zhunan Miaoli, Miaoli County, Taiwan
- 中文摘要:
- 英文摘要:
Background
Lytic reactivation of EBV has been reported to play an important role in human diseases, including NPC carcinogenesis. Inhibition of EBV reactivation is considered to be of great benefit in the treatment of virus-associated diseases. For this purpose, we screened for inhibitory compounds and found that apigenin, a flavonoid, seemed to have the ability to inhibit EBV reactivation.
Methods
We performed western blotting, immunofluorescence and luciferase analyses to determine whether apigenin has anti-EBV activity.
Results
Apigenin inhibited expression of the EBV lytic proteins, Zta, Rta, EAD and DNase in epithelial and B cells. It also reduced the number of EBV-reactivating cells detectable by immunofluorescence analysis. In addition, apigenin has been found to reduce dramatically the production of EBV virions. Luciferase reporter analysis was performed to determine the mechanism by which apigenin inhibits EBV reactivation: apigenin suppressed the activity of the immediate-early (IE) gene Zta and Rta promoters, suggesting it can block initiation of the EBV lytic cycle.
Conclusion
Taken together, apigenin inhibits EBV reactivation by suppressing the promoter activities of two viral IE genes, suggesting apigenin is a potential dietary compound for prevention of EBV reactivation. - 中文關鍵字:
- 英文關鍵字: Epstein-Barr virus, Apigenin, Reactivation, Nasopharyngeal carcinoma