- 作者: 何志?; 陳豪勇; 彭瑞貞; 賴竹蘭
- 作者服務機構: 榮民總醫院醫學研究部; 預防醫學研究所
- 中文摘要: 共選用18種不同型的白血病細胞株分別測定其對甲、乙、丙三種干擾素抗細胞及抗病毒作用的感受性。一般發現只有三種骨髓性白血病株U937,KG-1和HL-60對干擾素有較高感受性,而其他系列的細胞株則不太敏感。為了要測定細胞株對干擾素抗病毒作用的敏感度,首先測定各細胞是否能支持VSV,SBV,SFV病毒複製。意外地發現只有三種細胞-Raji,K562及U937對SFV病毒感受性較強,其他的細胞則對三種病毒均不敏感,用SFV病毒作為指標可測出三種敏感細胞株對各種干擾素的抗病毒作用均能表現,且效果之順序為甲種干擾素>乙種干擾素>丙種干擾素,實驗結果的意義將在文中討論。
- 英文摘要: A total of 18 different types of human leukemic cell lines were tested for their susceptibility to theanticellular and antiviral effects of interferons (IFNs) α, β andγ. In general, only the three myelogenousleukemic cell lines U937, KG-1 and HL-60 were found to be highly susceptible to the anticellular effect ofthe different IFNs while cells of the other lineages were relatively resistant. In order to determine whetherthe cell lines were sensitive to the antiviral effects of IFNs, the cells were first screened for their ability tosupport the replication of vesicular stomatitis virus (VSV), sindbis virus (SBV) and semliki forest virus(SFV). Unexpectedly, only three cell lines-Raji, K562 and U937 were highly susceptible to SFV whileother cell lines were relatively refractory to all three viruses. Using SFV as indicator virus, the antiviralactivity of all IFNs could be detected in all three cell lines and their relative efficiency was in the order ofα>β>γ. The significance of these results were discussed.
- 中文關鍵字: IFNs; leukemic cell lines; antiviral; anticellular
- 英文關鍵字: --