- 作者: Yi-Jen Hsueh; Der-Yuan Wang; Chien-Chia Cheng; Jan-Kan Chen
- 作者服務機構: Department of Physiology, College of Medicine, Chang Gung Univeersity, Taoyuan, Taiwan
- 中文摘要: --
- 英文摘要: In this study, we examined the postnatal expression pat-terns of p63 and other keratinocyte stem cell markers inthe rat cornea in an attempt to determine the markersthat best represent characteristics of corneal keratino-cyte stem cells. We show that the expression of p63 inthe rat cornea is unique and differs from that observed inhumans.It changes with age, from central cornea-posi-tive, peripheral cornea-positive, and limbus-positive, tocentral cornea-positive, peripheral cornea-positive, andlimbus-negative, and finally to central cornea-negative,peripheral cornea-positive, and limbus-negative, as ex-amined by immunohistochemical staining. However,when a more sensitive staining method was used,thelimbus was also shown to be positive for p63, indicatinga lower level of expression than that of the peripheralcornea.The basal layer of the rat limbal epithelium is thesite where (β-catenin+,K14+, PCNA-,and K3-cells reside.This cell layer is also the site where slow-cycling cells arelocated. In contrast with observations made in humans,our results clearly indicate that p63 is expressed in stemcells and young transient amplifying cells of the rat cor-nea, with higher levels of expression in the latter.
- 中文關鍵字: --
- 英文關鍵字: --