- 作者: Kai-Ping N. Chow, His-Chi Lu, Huar-Fen Chou, Hao-Ping Liu, Shie-Liang Hsieh, Yu-Sun Chang, Kong-Bung Choo
- 作者服務機構: Department of Microbiology and Immunology ,Medical College, Graduate Institute of Basic Medical Sciences, Chang Gung University, Taoyuan, Department of Medical Research, Changhua Christian Hospital, Changhua, Department of Medical Research and Education, Veterans General Hospital-Taipei, Department of Microbiology and Immunology, National Yang Ming University school of Medicine, Taipei, Taiwan, ROC
- 中文摘要: --
- 英文摘要: visually complete regression of the transplanted NPCtumor loads within 20 days after GCV treatment. Takentogether, results from this pilot study indicate the feasi-bility of the development of a gene therapeutic protocolbased on the chemosensitive gene constructs describedin this paper.Nasopharyngeal carcinoma (NPC) is a human cancer ofepithelial cell origin. Infection by Epstein-Barr virus hasbeen shown to be closely associated with this tumor.Recent studies have indicated that another common epi-theliotropic virus, human papillomavirus (HPV), is alsofound in a significant number of NPC cases. In this study,we evaluated the feasibility of using the HPV regulatorylong control region (LCR) to drive the expression of thethymidine kinase (tk) gene to achieve chemosensitivityfor gene therapeutic treatment of NPC. Testing HPV-11-LCR-tkconstructs in NPC cell lines in the presence of gan-ciclovir (GCV) led to 50-60% cell death of transfectedcells. The therapeutic efficacy was further tested in an invivo model using nude mice transplanted with tumorsderived from transfected NPC cells. Injection of 50 mg/kgbody weight GCV twice daily for 14 days resulted in
- 中文關鍵字: --
- 英文關鍵字: Gene therapy, Human papillomavirus, Nasopharyngeal carcinoma, Prodrug treatment, Thymidine kinase