• 作者： 陳進庭; 王智昱; 郭英雄; 江惠華; 周松男; 蕭伊芸; 江俊斌
• 作者服務機構： 國立台灣大學醫學院 雷射醫學研究中心 牙醫學系 婦產科; 國立陽明大學醫學工程研究所
• 中文摘要： 口腔癌目前已位居台灣地區十大癌症之一，近年來國人因嚼食檳榔而引發的口腔癌案例有愈來愈多的趨勢，因此發展一快速且簡單的口腔癌早期診斷方法有其必要性。本研究擬對“光激發螢光光譜”作為口腔癌早期診斷工具的可行性作探討。從270 nm至310 nm的激發光全光譜分析結果中，我們發現正常口腔黏膜和口腔癌組識於330 nm和470nm左右的波峰位置有明顯的差異性存在。而於所用的激發光波長中，又以300 nm的波長最能呈現出口腔正常組識和病變組識的差異性。所有待測的口腔組識在經由300 nm的光激發後，我們對其螢光光譜中330 nm和470 nm左右的波峰強度做一簡單的數學比值分析。結果發現93%以上的正常口腔黏膜其比值皆低於17，而94%的口腔癌或癌前病變組織其比值皆高於17。這一結果顯示對口腔組識做300 nm波長的光激發螢光光譜測量，其結果可作為診斷口腔病變及選擇活體切片位置之參考。
• 英文摘要： For the early detection of oral neoplasia, light-induced fluorescence spectroscopy was used to measurethe fluorescence emission of malignant (squamous cell carcinoma & verrucous carcinoma) and premalignant(epithelial dysplasia, hyperkeratosis & lichen planus) oral tissues as well as normal oral mucosa ex vivo toassess the ability of this technique to distinguish neoplastic from normal oral tissues. The emission spectra of histologically normal and neoplastic oral tissues were obtained under excita-tion wavelengths varied from 270 nm to 400 nm at 10-nm intervals. At 300-nm excitation, the most intenselyfluorescent peak occurred at 330-nm and 470-nm emission. At 330-nm emission, the spectrum of the malig-nant oral tissue was significantly stronger than that of the normal oral mucosal tissue after area normalization.However, at 470-nm emission, the spectrum of the malignant oral tissue was significantly weaker than that ofthe normal oral mucosal tissue. A diagnostic algorithm based on the ratio of relative intensities of 330 nm to470 nn emission within the ±5 run peak area of each sample was calculated and paired. The histogram ofratios showed that histologically neoplastic oral tissues could be distinguished from normal oral mucosal tis-sues using the 300 nm excitation wavelength. The average ratio of malignant or premalignant oral sampleswas significantly greater than that of the normal oral mucosal samples (p < 0.001). This ex vivo study indicatedthat fluorescence spectroscopy may be useful in differentiating malignant or premalignant oral tissue fromnormal oral mucosa.
• 中文關鍵字： oral cancer; light-induced fluorescence spectroscopy.
• 英文關鍵字： --