• 作者： 劉雨田; 嵇達德; 周俊吉; 趙海淵; 廖經倫; 韓韶華
• 作者服務機構： 國防醫學院微生物及免疫學研究所; 國立陽明醫學院微生物及免疫學研究所
• 中文摘要： E. coli ATCC 9637之盤尼西林醯?基因（pac基因）已被分離。它可提供E. coli HB 101及其它腸道桿菌生產盤尼
  西林醯?的能力。核酸限制?分析及基因再選殖的結果揭露該基因係被包含於一段2.3 kb之HindⅢ-Sma I之DNA
  片段。細胞外蛋白質合成研究結果顯示該基因產物為一大約90 kDa之蛋白質。pac基因可在不同的宿主細胞中表現，且
  盤尼西林醯?活性的產能被改善。應用含有開放表現之pac基因之重組質體進行基因工程改造腸道桿菌，可使其盤尼西
  林醯?活性的產量比原生產菌株，E. coli ATCC 9637，提高二十倍以上。
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• 英文摘要： The penicillin G acylase gene (pac gene) from Escherichia coli ATCC 9637 has been isolated. It con-ferred the production of penicillin G acylase upon E. coli HB101 and other enteric bacilli. Restrictionenzyme analysis and subcloing studies reveal that the gene is contained within a 2.3 kb HindⅢ-SmaI DNAfragment. In vitro protein synthesis study suggests a gene product of approximately 90 kDa. By using thegenetically engineered bacteria which harbor a novel recombinant plasmid (pGL5) bearing a constitutivelyexpressible pac gene, a 20-fold increased production yield of penicillin G acylase activity was obtained ascompared with that produced by the original strain, E. coli ATCC 9637.
• 中文關鍵字： penicillin G acylase; pac gene; enteric bacilli; stability; in vitro protein syntheseis.
• 英文關鍵字： --