- 作者: Mohamed D Morsy; Ossama A Mostafa; Waleed N Hassan
- 作者服務機構: Physiology Department, College of Medicine, Menoufiya University, Egypt
- 中文摘要: --
- 英文摘要:
Background: Paraplegia remains a potential complication of spinal cord ischemic reperfusion injury (IRI) in
which oxidative stress induced cyclooxygenase activities may contribute to ischemic neuronal
damage. Prolonged administration of vitamin E (α-TOL), as a potent biological antioxidant, may
have a protective role in this oxidative inflammatory ischemic cascade to reduce the incidence of
paraplegia. The present study was designed to evaluate the preventive value of α-TOL in IRI of
spinal cord.
Methods: For this study, 50 male Sprague-Dawley rats were used and divided into five experimental groups
(n=10): Control group (C); α-TOL control group (CE) which received intramuscular (i.m.) α-TOL
injections (600 mg/kg); Sham operated group (S), IRI rats were subjected to laparotomy and
clamping of the aorta just above the bifurcation for 45 min, then the clamp was released for 48 hrs
for reperfusion; and IRIE rats group, received 600 mg/kg of α-TOL i.m. twice weekly for 6 weeks,
followed by induction of IRI similar to the IRI group. At the end of the experimental protocol;
motor, sensory and placing/stepping reflex evaluation was done. Plasma nitrite/nitrate (NOx) was
measured. Then animals’ spinal cord lumbar segments were harvested and homogenized for
measurement of the levels of prostaglandin E2 (PGE2), malondialdehyde (MDA) and advanced
oxidation products (AOPP), while superoxide dismutase (SOD) and catalase (CAT) activity were
evaluated.
Results: Induction of IRI in rats resulted in significant increases in plasma levels of nitrite/ nitrate (p<0.001)
and spinal cord homogenate levels of PGE2, MDA, advanced oxidation protein products AOPP and
SOD with significant reduction (p<0.001) in CAT homogenate levels. Significant impairment of
motor, sensory functions and placing /stepping reflex was observed with IRI induction in the spinal
cord (p<0.001). α-TOL administration in IRIE group significantly improved all the previously
measured parameters compared with IRI group.
Conclusions: α-TOL administration significantly prevents the damage caused by spinal cord IRI in rats with
subsequent recovery of both motor and sensory functions. Alpha-tocopherol improves the oxidative
stress level with subsequent reduction of the incidence of neurological deficits due to spinal cord IRI
conditions. - 中文關鍵字: --
- 英文關鍵字: --