- 作者: 趙大衛; 黃璧祈; 陳韻安; 王仕蓉
- 作者服務機構: 國立陽明醫學院寄生蟲學科; 國立臺灣師範大學生物學系
- 中文摘要: 本研究之目的係嘗試以吾人設計之多環純化管組合,由生物檢體中迅速有效的純化分離原生鞭毛蟲。研究之第一部分,使用已在實驗室長期繼代培養之人類株腸道鞭毛蟲(Pentatrichomonas hominis)。將每毫升2x10 的蟲體與同體積每毫升2x10 的大腸桿菌混合,於多環管組合中以含有適當量抗生素之Trypticase yeast extract iron-serum-33培養基在37℃培養。每毫升1x10 無細菌污染之鞭毛蟲可於四天後在單環管及雙環管中出現;在三環管中則需六天;傳統之U-型管無法防止細菌污染。第二部分研究使用由臺灣獮猴所採得之糞便,加入適當量之抗生素後於多環管組合中分離,經證實五天培養後在雙環管中可得大量無其他微生物污染之腸道鞭毛蟲。此種多環純化管組合,可應用於其他種類運動活潑之鞭毛蟲的分離及純化培養上。
- 英文摘要: It is always troublesome having protozoan cultures contaminated with other organisms in the labora-tory. The method described here produces high efficiencies of purification for fast moving flagellate pro-tozoa. A human strain Pentatrichomonas hominis was employed in the study to examine the effects ofmultiple loop tubes on the purification of flagellates. Trichomonads were harvested from a trypticase yeastextract iron-serum-33 (TYI-S-33) medium, adjusted to 2 x 10 organisms/ml, and mixed with an equalvolume of 2 x 10 organisms/ml of bacteria. The isolation was performed at 37℃ in TYI-S-33 medium con-taining a suitable amount of antibiotics (1000 U/ml of penicillin, 1000 μg/ml of streptomycin, and 4 μg/mlof fungizone). Four days later, 10 organisms/ml of protozoa, free of bacteria, were observed at the otherend of the single loop and the double loop tubes. About the same amount of flagellates could be found atthe other end of the triple loop tube six days after incubation. The traditional U-shaped tubes were used ascontrols and 10 cells/ml of flagellates were recovered in the presence of bacteria two clays after incubation.An axenic culture of P. hominis was successfully isolated from the feces of a Formosan rock-monkey,Macaca cyclopsis, by this method. Purified trichomonads were recovered from a double loop purificationtube five days after incubation.
- 中文關鍵字: Pentatrichomonas hominis; axenic cultivation; multiple loop tubes; purification; in vitro
- 英文關鍵字: --