- 作者: Manas k. Majumdar Michele Keane-Moore Diana Buyaner Wayne B. Hardy Mark A. Moorman Kevin R. Mclntosh Joseph D. Mosca
- 作者服務機構: Osiris Therapeutics, Inc., Baltimore, Md., USA
- 中文摘要: --
- 英文摘要:
We have characterized adhesion molecules on the sur-
face of multipotential human mesenchymal stem cells
(hMSCs) and identified molecules whose ligands are
present on mature hematopoietic cells. Flow cytometric
analysis of hMSCs identified the expression of integrins:
α1,α2,α3,α5,α6,αv,β1,β3, and β4, in addition to
ICAM-1, ICAM-2, VCAM-1, CD72, and LFA-3. Exposure of
hMSCs to lL-1α, TNFα or IFNγ up-modulated ICAM-1 sur-
face expression, whereas only IFNγ increased both HLA-
class I and -class II molecules on the cell surface. Whole
cell-binding assays between the hMSCs and hemato-
poietic cell lines showed that T lymphocytic lines bound
hMSCs with higher affinity than lines of either B lympho-
cytes or those of myeloid lineage. Experiments using
autologous T lymphocytes isolated from peripheral
blood mononuclear cells showed that hMSCs exhibited
incleased affinity for activated T-lymphocytes compared
to restion T cells by quantitative whole cell binding and
rosetting assays. Flow cytometric analysis of rosetted
cells demonstrated that both CD4+ and CD8+ cells bound
to hMSCs. To determine the functional significance of
these findings, we tested the ability of hMSCs to present
antigen to T lymphocytes. hMSCs pulsed with tetanus
toxoid stimulated proliferation and cytokine production
(lL-4, lL-10, and lFNγ) in a tetanus-toxoid-specific T cell
line. Maximal cytokine production correlated with maxi-
mal antigen-dependent proliferation. These data demon-
strate physiological outcome as a consequence of inter-
actions between hMSCs and human hematopoietic li-
neage cells, suggesting a role for hMSCs in vivo to
influence both hematopoietic and immune function(s). - 中文關鍵字: --
- 英文關鍵字: Mesenchymal stem cells. Antigen presentation. Tlymphocytes. Hematopoietic interactions. Immune function