- 作者: Chun-Hsien Yu; Hui-Ling Chen; Wei-Tsung Chen; Yen-Hsuan Ni; Yah-Luen Lin; Mei-Hwei Chang
- 作者服務機構: Graduate Institute of Clinical Medicine, Department of Pediatrics, Hepatitis Research Center, Laboratory Animal Center, National Taiwan University Hospital and National Taiwan Universty College of Medicine, Department of Pediatrics, Taipei Municipal Women's and Children's Hospital, and Department of Radiology, Taipei Municipal Jen-Ai Hospital, Taipei, Taiwan
- 中文摘要: --
- 英文摘要: Hepatocyte transplantation has been proposed as analternative for rescuing patients with acute hepatic fail-ure. However, portal hemodynamic changes and issuesof safety after hepatocyte transplantation in acute hepat-ic failure have not been systemically evaluated becauseof the lack of a suitable experimentation system.In thisstudy, we created a novel spring-guidewire introducerneedle to simplify the technique for long-term portal can-nulation in F-344 rats. The portal cannula was capable ofbeing used for blood sampling, infusion of hepatocytes,and measurement of portal hemodynamic changes. Oneweek after portal cannulation, rats were injected with D-galactosamine(1.35 g/kg, i.p.)to induce hepatic failure.Hepatocytes(2 x 10)were infused intraportally 24-26 hafter induction of liver injury. Portal pressures wererecorded for up to 60 min after hepatocyte transplanta-tion. Intraportal infusion of 2 x 10 hepatocytes causedan instantaneous onset of portal hypertension. The mag-nitude of the rise in portal pressure was similar in bothnormal rats and rats with acute hepatic failure(33.0 +7.1 vs. 37.7 + 0.5 mm Hg;p=0.23). However, the resolu-tion rate of portal hypertension was remarkably delayedin rats with acute hepatic failure, and the portal pressurewas significantly higher than that in normal rats 60 minafter hepatocyte transplantation (25.0+2.8 vs. 14.5+2.4 mm Hg; p=0.007). In conclusion, we have estab-lished a simple new technique for long-term portal can-nulation of rats. Our studies provide critical insights intothe delayed resolution of portal hemodynamics afterhepatocyte transplantation in subjects with acute hepaticfailure.
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- 英文關鍵字: --