- 作者: 劉二保,衛洪清
- 作者服務機構: College of Chemistry and Life Sciences, Tianjin Normal University, Tianjin 300074, P. R. China
- 中文摘要: A new method for the determination of baicalin in Scutellariae Radix extract and its preparations by high performance liquid chromatography (HPLC) coupling with flow injection chemiluminescence detection (FIA-CL) has been developed. The method was based on the chemiluminescence reaction of potassium permanganate with baicalin in nitric acid medium; the CL intensity can be enhanced by formaldehyde. In this study, the conditions of chemiluminescence and chromatography were examined, and the schematic diagram of the HPLC-FIA-CL analyzer was optimized. The analytes were separated on Hypersil RP-C18 columns (100 ’ 4.6 mm, I.D., 5 mm) by equality elution with 47:53 (v/v) methanol-0.3% phosphoric acid as a mobile phase at a flow rate of 0.8 mL·min-1 and a column temperature of 40 °C. Under the optimum condition, the CL intensity was proportional to the concentration of baicalin over the range of 4.10 ’ 10-7 ~ 6.15 ’ 10-5 mol·L-1. The limit of detection (S/N = 3) was 2.79 ’ 10-7 mol·L-1 with the relative standard deviation 2.5% (Cs = 6.15 ’ 10-6 mol·L-1, n = 5). The method has been applied to the determination of baicalin in Scutellariae Radix extract and its preparations, and satisfactory results were obtained.
- 英文摘要: --
- 中文關鍵字: HPLC; Flow injection; Chemiluminescence detection; Baicalin; Potassium permanganate.
- 英文關鍵字: --