第6卷‧第4期,
198210
, pp. 411-415
β-Bungarotoxin還原後之氧化重組
- 作者:
陳長瑞; 吳世雄; 何純郎; 王光燦
- 作者服務機構:
中央研究院生物化學研究所
- 中文摘要:
β-Bangarotoxin在含有 6M 鹽酸胍的溶液中,經由2- 硫酸乙醇的還原作用將它所具有的十對雙硫鍵全部打斷,致使構成此毒素的兩個次單元也因雙硫鍵的打斷而分開,同時失去其原有構造及生物活性。此兩個分開之次單元在除去還原劑後,可在半胱氨酸的存在下被再氧化而重組成一個具有生物活性的分子。再氧化重組的β-bangarotoxin經過柱狀盡析純化後,回收百分之二十五的毒素,它們不論是生物活性、電泳性、抗原性以及 CD 光譜都和原來的毒素完全相同。
- 英文摘要:
Reduction of β-bungarotoxin with 2-mercaptoethanol in the presence of 6 M guanidine hydroch-loride resulted in a complete disruption of all ten disulfide linkages and the loss of its biological activity.Two distinct dissimilar subunits obtained after complete reduction could be reoxidized in the presence of5 mM cysteine and recombined to form a functional active single molecule having phospholipase activityand neuromuscular blocking action. After purification, the reoxidized β-bungarotoxin was recovered in25% yield. The reoxidized β-bungarotoxin has the same CD spectrum, electrophoretic mobility and anti-genisity as the native β-bungarotoxin.
- 中文關鍵字:
--
- 英文關鍵字:
--