- 作者: Edward D. Blair ; Christopher M. Roberts ; B. Wendy Snowden ; Anne Gatignol ; Monsef Benkirane ; Kuan-Teh Jeang
- 作者服務機構: a Wellcome Research Laboratories, Beckenham, UK; b Molecular Virology Section, Laboratory of; b Molecular Microbiology, NIAID, NIH,Bethesda, Md., USA
- 中文摘要: --
- 英文摘要: TAR RNA-binding protein TRBP was originally isolated by its binding affinity for radiolabeled HIV-1 leader RNA. Subsequent studies have suggested that this protein is one member of a family of double-stranded RNA-binding proteins. Recent findings indicate that TRBP might function to antagonize the translational inhibitory effect that can be mediated through cellular protein kinase, PKR. Here, we report on the over-expression of a cDNA coding for TRBP in eukaryotic SF9 cells using baculovirus. We characterized the nuclear localization of TRBP in insect cells, and we demonstrate that TRBP co-immu-noprecipitates with a protein in these cells antigenically related to human PKR.
- 中文關鍵字: --
- 英文關鍵字: HIV-1 ; Tat; RNA-binding protein; TRBP; TAR RNA; PKR