- 作者: 林仁混; 吳尚修; 陳錦翠
- 作者服務機構: 國立臺灣大學醫學院生化學研究所
- 中文摘要:
食物中含有之胺類化合物已被認為可能產生致癌性亞硝基化合物之前驅物質。最近日本科學家從醬油中分離出一種
稱為MTCA(1-Methyl-1, 2, 3, 4-Tetrahydro-2-Carboline-3-Carboxylic Acid)的化合物,經過亞硝酸鹽處理後可產生致
突變物質。基他?卜?(Carboline)衍生物如harman,norharman,harmaline,harmalol,harmine 及 harmol之致突變作用
因而引起我們研究的興趣。這些化合物與MTCA一樣,經亞硝化作用之後可產生較強的致突變物質。MTCA之去甲基衍
生物TCA(1, 2, 3, 4-Tetrahydro-2-Carboline-3-Carboxylic Acid)經亞硝基化後也變為較強的致突變物質。由色胺酸加
熱產生之強致突變化合物Trp-P-2經亞硝化作用後,其致突變力反而下降。MTCA經亞硝化作用後可產生數種衍生物,
經分離後可直接對沙門菌(TA 98)有致突變作用,這些亞硝基化合物之構造正在研究當中。
B - 英文摘要: Food-borne amines have been considered as the potential precursors of endogenous carcinogenic N-nitroso compounds in humans. A compound which yields a direct mutagen after nitrite treatment wasisolated from soy sauce and was identified as 1-methyl-1, 2, 3, 4-tetrahydro-2-carboline-3-carboxylic acid(MTCA) (Wakabayashi, et al., 1983). The mutagenicities of other carboline derivatives such as harman,norharman, harmaline, harmalol, harmine, and harmol were studied. Like MTCA, the nitrosated carbolinederivatives showed higher mutagenic activity as compared to their corresponding parent compounds. Thedemethylated analogue of MTCA, 1, 2, 3, 4-tetrahydro-2-carboline-3-carboxylic acid was synthesized and itsnitrosated products were shown to be mutagenic to Salmonella typhimurium TA 100 and TA 98. Thepotent mutagen Trp-P-2 is a typical 3-carboline derivative. The mutagenicity of Trp-P-2 was suppressedremarkably after nitrosation. Several 3-carboline derivatives also showed the similar property. Nitrosationof MTCA gave several derivatives which were isolated and showed direct mutagenicity to Salmonella typhi-murium TA 98. Further characterization of these new carboline derivatives is in progress.
- 中文關鍵字: 2-carbolines; 3-carbolines; mutagenicity; nitrosation
- 英文關鍵字: --