- 作者: Kuo-Hsiung Huang, Chun-Hua Wang, Chien-Huang Lin, Han-Pin Kuo
- 作者服務機構: Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan R.O.C
- 中文摘要: --
- 英文摘要:
Background:
Our previous study showed NF-κB repressing factor (NKRF) downregulates IP-10 and IL-8 synthesis in the peripheral blood mononuclear cells and alveolar macrophages of TB patients with high bacterial loads. However, the mechanism underlying the repressive effect of NKRF is not fully understood.
Results:
The levels of IP-10, IL-8 and NKRF were significantly up-regulated in THP-1 cells treated with heated mycobacterium tuberculosis (H. TB). NKRF inhibited NF-κB-mediated IP-10 and IL-8 synthesis and release induced by H. TB. The epressive effect of NKRF is mediated via interference with NF-κB (p65) binding and RNA polymerase II recruitment to promoter sites of IP-10 and IL-8.
Conclusions:
We have elucidated that direct contact with MTb induces IP-10, IL-8 and a concomitant increase in NKRF in THP-1 cells. The up-regulated NKRF serves as an endogenous repressor for IP-10 and IL-8 synthesis to hinder host from robust response to MTb infection. - 中文關鍵字: --
- 英文關鍵字: Tuberculosis, IP-10, IL-8, NF-κB repressing factor