- 作者: 李建平,顧海寧
- 作者服務機構: Department of Material and Chemistry Engineering, Guilin University of Technology, Guilin 541004, P. R.China
- 中文摘要: An amperometric cholesterol biosensor based on immobilization of cholesterol oxidase in a Prussian blue (PB)/polypyrrole (PPy) composite film on the surface of a glassy carbon electrode was fabricated. Hydrogen peroxide produced by the enzymatic reaction was catalytically reduced on the PB film electrode at 0 V with a sensitivity of 39 μA (mol/L)-1. Cholesterol in the concentration range of 10-5 ~ 10-4 mol/L was determined with a detection limit of 6 ’ 10-7 mol/L by amperometric method. Normal coexisting compounds in the bio-samples such as ascorbic acid and uric acid do not interfere with the determination. The excellent properties of the sensor in sensitivity and selectivity are attributed to the PB/PPy layer modified on the sensor.
- 英文摘要: --
- 中文關鍵字: Biosensor; Cholesterol oxidase; Prussian blue; Polypyrrole; Amperometry.
- 英文關鍵字: --