- 作者: 陳?紀
- 作者服務機構: 國立中興大學
- 中文摘要:
將水稻黃萎病、水稽草狀矮化病、甘藷簇葉病、大豆簇葉病、牛筋草叢葉病、甘蔗白葉病、狗
牙根白葉病、四生臂形草白葉病、香蕉萎縮病、柑桔黃龍病及蘆筍萎黃病等十一種叢生萎黃病類
之罹病葉,行電子顯微鏡觀察之結果,除香蕉萎縮病外,均於其?皮部(主為篩管)內發現多數
之大小不一而多形性之Mycoplasma粒子。此等粒子外圍具有單位膜,但無細胞壁。其內部充滿
ribosome顆粒及類似DNA之網狀絲。ribosome顆粒一般多位於粒子之周邊,而網狀絲則位
於中心較為室虛之部位。於香蕉萎縮病之場合,Mycoplasma則僅見於表皮細胞而不能觀察於?
皮部細胞。此種差異可能因媒介昆蟲之吸液方式不同所致者。
於所有之病例,其大小不一之Mycoplasma粒子混生於同一細胞內,由此可推測Mycopl-
asma乃由小型粒子漸次生長變為大型粒子者。又有些觀察顯示成熟粒子以芽殖方式或裂殖方式增
殖之情形。另於柑桔黃龍病之場合觀察到大型粒子內含有數個基本小體之情形。此等觀察均為暗示
Mycoplasma生活環之幾個端面。
於帶毒之甘蔗白葉病媒介昆蟲(臺灣斑紋浮塵子)之消化管細胞內亦發現多數之Mycoplasma。
其形態與甘蔗病葉內者略同。本觀察乃支持Mycoplasma為植物病原體之一有力傍證。
e - 英文摘要: Electron microscopic examinations of the preparations from young leaves infected with rice yellow dwarf, rice grassy stunt, sweet potato witches' broom, soybearwitches' broom, Eleusine indica witches' broom, sugarcane white leaf, Cynodon dactylorwhite leaf, Brachiaria distachya white leaf, banana bunchy top, citrus likubin andasparagus yellow dwarf revealed the presence of pleomorphic bodies which resembledMycoplasma species. These Mycoplasma-like organisms were found in various sizeand varying concentration in sieve elements of vascular tissues except bananabunchy top which were found in the epidermal cells. These bodies were possesecof a two-layered unit membrane. The cytoplasm of the Mycoplasma-like bodies werefilled with ribosome granules and DNA-like net-strands. The former were distributedaround the periphery and the later were located in the central vacuolated area. Occasionally, budding-like protrusion from the large body, constriction at themiddle of the large bodies, and some discernible“elementary bodies" within a largebody were obserbed which were suggestive of multiplication of new bodies. Thediversity in size of the bodies found simultaneously in one cell might representtheir developing stages. Sections of intestine of leafhopper vector Matsusmuratettix hiroglyphicus, whichhad been exposed to sugarcane white leafe-affected plants, revealed also thepresence of Mycoplasma-like organisms similer to those which were observed ininfected plant tissues. This finding might support the idea that the Mycoplasmacould be one of the plant disease principles.
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- 英文關鍵字: --