- 作者: Lung-Ji Chang, Chih-Hsiung Chen; Vicki Urlacher; Tze-Fun Lee
- 作者服務機構: Department of Molecular Genetice and Microbiology, Gnen Therapy Center and Brain Institute, University of Florida, Gainesville, Fl., USA; Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Canada
- 中文摘要: --
- 英文摘要: The growth inhibitory effects of Vpr and Vpx are species-and cell type-dependent. HIV-1,HIV-2 and SIV Vpr areprimarily cytostatic in mammalian cells and HIV-1 Vprhas been reported to induce apoptosis in human cells.Our previous studies have shown that HIV-1, HIV-2 andSIV Vpr and Vpx have differential cytostatic and cytotoxiceffects in the yeast cells [Zhang et al.: Virology, 230:103-112; 1997]. Here, we further examined the apoptosisfunction of HIV-1 Vpr in different species of mammaliancells and investigated if other primate lentiviral Vpr andVpx exert similar functions. Our results show that noneof the primate lentiviral Vpr or Vpx we tested inducesapoptosis in nonhuman species of mammalian cells.However, HIV-1 Vpr, but not HIV-2 or SIV Vpr and/or Vpx,induced apoptosis in different types of human cell lines.Further, the apoptotic effect of HIV-1 Vpr can be distin-guished from that of the human interferon-r, a knownproapoptotic protein, that HIV-1 Vpr shows little to noparacrine and/or bystander effect. When coexpressedwith Bcl-2 or Bcl-XL, the apoptotic effect of HIV-1 Vprbecame markedly attenuated. These results indicate thatthe apoptotic effect of HIV-1 Vpr is species-dependentand is intracellularly modulated by the Bcl-2 family ofproteins. Our study also suggests that the proapoptoticfunction of HIV-1 Vpr is developmentally associated withhuman but not nonhuman primate species.
- 中文關鍵字: --
- 英文關鍵字: --