- 作者: Z.N. Chang; C.C Liu ; H.C Perng; L.C. Tsai; S.H Han
- 作者服務機構: a Shool of Medical Technology and Institute; of Microbiology and Immunology, National Yang-Ming Medical College, and; b Department of Medical Research, Veterans General Hospital-Taipei, Taipei, Taiwan, Republic of China
- 中文摘要: --
- 英文摘要: The present study disclosed the cross-reactivity between Bermuda grass pollen (BGP) and other grass pollens using monoclonal antibodies (MAbs) and poly- clonal antiserum. MAb 9-13, directed against a group of minor allergens of BGP (Cyn d Bd68K, 48K, 38K) was found to cross-react with extracts of ten other grass pollens. Immunoblotting assays illustrated that MAb 9-13 cross- reacted with multiple components of most of these pollens, and the major cross-reactive components had molecular weights of 29-36 kD. The cross- reactivity between BGP and Lol p I, the group I allergen of rye grass pollen, was further evaluated; Lol p I was recognized by MAb 9-13, but not by our MAbs/polyclonal antiserum against Cyn d I, the major allergen of BGP. These results suggest that the epitope recognized by MAb 9-13 is a common (C) epi- tope shared by Lol p I and Cyn d Bd68K, 48K, 38K, and Cyn d I does not share significant antigenicity with Lol p I. In a modified radio-allergosorbent test, IgE antibodies in the serum of BGP-allergic patients reacted mildly with C- epitope-bearing components of both BGP and rye grass pollens, and this bind- ing could be blocked specifically by MAb 9-13. This suggests that in addition to an antigenic cross-reaction, the C epitope can also lead to an allergenic cross-reaction.
- 中文關鍵字: --
- 英文關鍵字: Common allergenic epitope; Minor allergen; Bermuda grass pollen; Cyn d I; Lol p I