• 作者： 畢萬邦; 林建輝
• 作者服務機構： 國立臺灣大學醫學院生理學科
• 中文摘要： 本報告為用卵巢初除鼠再處理卵巢激素，來觀察其引發黃體生成激素釋放的能力。 Sprague-Dawley種系的成年雌鼠，第一組：在動情期上午切除卵巢，隨即在皮下植一含雌性素的膠囊及一心房導管，以便於採血。第二組：也在動情期上午切除卵巢，但到次日（頂期的間情期第一日）上午，再植含雌性素膠囊及心房導管。第三組：在間情期第一日上午切除卵巢，隨即植一含雌性素的膠囊及心房導管。在預期的間情期第二日上午九時半，將上述各組雌鼠的一半處理橄欖油，另一半處理助孕素。當日下午一時、三時、和五時分別經心房導管採血分析血中黃體生成激素含量。結果顯示，有雌性素膠囊但只處理橄欖油的雌鼠中，第一組、第二組鼠血中黃體生成激素沒有顯著變化。而第三組有百分之八十的雌鼠在五時前後，血中黃體生成激素含量升高到達潮放的水準。另外凡接受助孕素的三組雌鼠皆呈類似排卵前黃體生成激素的潮放。結果說明在生殖週期中某段時期切除卵巢，雌性素本身即具有引發黃體生成激素釋放的能力。但經雌性素打底的卵巢切除鼠，助孕素能有效的增加黃體生成激素的大量釋放。
• 英文摘要： The capability of estradiol or and progesterone in inducing luteinizing hormone (LH)surge in acutely ovariectomized (Ovx) rats was studied. In group I, rats were Ovx on estrus and wereimplanted with capsules and atrial cannulae immediately after operation for blood samplings. In groupII, rats were also Ovx on estrus but were implanted with capsules and sampling cannulae the next day(the expected diestrus day 1, D1). In group III, rats were Ovx on D1, and were implanted with capsulesand atrial cannulae immediately after operation. All surgical operations were done around 1000h in themorning. On the expected diestrus day 2(D2) at 0930h, one half of the rats in each group received an oilvehicle or 2mg of subcutaneously. Blood samples were taken from the indwelled cannulae at 1300, 1500,and 1700hrs in the afternoon. Results showed that treatment amplified LH release in all three groups ofrats primed with and that the oil vehicle did not assist in LH release in primed rats of group I andgroup II, but it did in 8 out of 10 rats in group III in the late afternoon of D2. Results suggested that theestradiol alone was capable in inducing LH surge on the expected D2 afternoon, and that under estradiol-primed conditions, can trigger neural initiators to advance LH surge, but that the internal hormonalmilieu at the time of ovariectomy may affect the influence of ovarian steroids in inducing LH release.
• 中文關鍵字： LH; estradiol; progesterone; acutely ovariectomized rat.
• 英文關鍵字： --