- 作者: Bor-Luen Chiang; Pei-Ming Yang; Lih-Hwa Hwang ; Jo-Man Wang; Shing-Fen Kao; Chien-Hsiung Pan; Wei-Kuang Chi; Pei-Jer Chen; Ding-Shinn Chen
- 作者服務機構: a Graduate Institute of Clinical Medicine, ; b Department of Internal Medicine, ; c Hepatitis Research Center, College of Medicine, National Taiwan University; ; d Process Development Division,Development Center for Biotechnology,Taipei, Taiwan ROC
- 中文摘要: --
- 英文摘要: Our previous study showed dominant proliferative response of peripheral mononuclear cells to hepatitis C virus (HCV) nonstructural (NS-3) (T9, from aa 1188 to 1493) in chronically infected patients. Six T9-specific T-cell clones derived in an HCV patient were established and studied for the antigen specificity and the ability of augmentation of in vitro antibody production. All these cloned T-cell lines responded exclusively to T9 antigen and could help autologous B cells in producing anti-T9 antibody in vitro. Cytokine mRNAs of these T cells was detected by polymerase chain reaction and predominant IL-2 and IFN-γ production was noted. In addition, further elucidation of T-cell antigenic determinant and MHC restriction suggested that these T-cell clones recognized at least two different T-cell antigenic determinants within the NS-3 region in an HLA DQ2-restricted manner. We believe characterization of HCV-specific T-cell responses, especially T-cell epitope mapping and cytokine production pattern, may shed light on further understanding the pathogenic mechanism and designing therapy for HCV infection.
- 中文關鍵字: --
- 英文關鍵字: Hepatitis C virus ; T-cell clones ; Cytokines