- 作者: 楊照雄;杜鳳吟;歐晉義;楊錫銘
- 作者服務機構: 國立臺灣大學醫學院微生物學研究所; ?生署預防醫學研究所
- 中文摘要: Epstein-Barr (EB)病毒是親淋巴細胞病毒而僅能感染B淋巴細胞,但最近之研究顯示鼻咽癌(NPC)之上皮細胞內有 EB病毒之基因。?明瞭EB病毒如何進入上皮細胞,將三種人類上皮細胞(KB, HeLa及BL-1)及三種猴細胞株(GMK, TMT-5及TM-9)藉仙臺病毒之助和含有EB病毒基因之Burkitt淋巴瘤細胞株P3HR-1及Raji細胞融合。融合細胞以 5-iodo-2'-deoxyuridine (1UdR)及低溫(32℃)培養處理後用螢光抗體檢驗法(FA法)測定EB病毒外殼抗原(Viral caps?d antigens, VCA)及早期抗原(Early antigens, EA)。另一方面?提高細胞對EB病毒之感受性,以Trypsin EDTA (ethylene-diamine-tetraacetic acid), DEAE-dextran及1UdR處理KB, HeLa, BL-1及TM-9細胞,然後以EB病毒 感染並用FA法測定VCA及EBV-associated nuclear antigens (EBNA)。所得結果簡述如下: (1) HeLa/P3HR-l及HeLa/Raji融合細胞以1UdR處理後分別有VCA及EA出現。 (2) TM-9/P3HR-1及TM-9/Raji融合細胞以1UdR處理後有VCA及EA出現。 (3) 預先以EDTA (0.75及1%) , DEAE-dextran (400μg/ml)及1UdR (120μg/ml)處理BL-I細胞然後感染EB 病毒則可測出EBNA及VCA 。
- 英文摘要: Epstein-Barr virus (EBV) is lymphotropic and only B-Iymphocytes have been known to be sensitive to EBV. But recent studies indicated that EBV genome was present in epithelial cells of nasopharyngeal carcinoma (NPC). Therefore, for understanding if EBV can enter epithelial cells, three human epithelial cell lines (KB, HeLa, and BL-1) and three monkey cells lines (GMK, TMT-5 and TM-9) were fused with EBV genome-carrying P3HR-1 and Raji cells by the aid of UV-inactivated Sendai virus. The fused cells were treated with 5-iodo-2'-deoxyuridine (1UdR) or incubated at low temperature (32°C) and then tested for EBV viral capsid antigens (VCA) and early antigens (EA) with the indirect immunofluorescent antibody technique. Moreover, KB, HeLa, BL-1, and TM-9 cells were treated with trypsin (0.025-0.2%), ethylene-diamine-tetraacetic acid (EDTA, 0.25-1%), DEAE-dextran (50-400μg/ml) or 1UdR (60-120μg/ml) and infected with EBV. The infected cells were tested for EBV VCA and EBNA (EBV-associated nuclear antigens). The results were summarized as follows: (1) EBV VCA and EA were detected in 1UdR-treated HeLa/P3HR-l and HeLa/Raji fused cells, respectively. (2) Both VCA and EA were observed in 1UdR-treated TM-9/P3HR-1 and TM-9/Raji fused cells. (3) Both EBNA and VCA were found in BL-1 cells treated with EDTA (0.75 and 1%), DEAE-dextran (400μg/ml) or IUdR (120μg/ml).
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