第6卷‧第8期,
197808
, pp. 789-795
乳山羊胚胎經過兔輸卵管移植於臺灣種山羊子宮之研究
- 作者：
林安仲
- 作者服務機構：
國立臺灣大學農學院獸醫學系
- 中文摘要：
將1～4細胞期之Saanen種乳山羊胚胎，由兔輸卵管腹腔端口注入輸卵管內。一至三日後採同，移植於與胚胎供者同期發情之臺灣種山羊子宮使之繼續發育至出生。出生後並以此代孕山羊哺乳。 注入兔輸卵管之胚胎，如管內液體飽滿，則能繼續細胞分裂。注入時為2細胞期之三個胚胎中之一個，於48小時後採同時在16細胞期。移植於受者後發育成為初生體重2.3公斤之正常牡仔羊。另二個於72小時後採同時在32細胞期。受者於移植後第115日解剖時懷有正常發育、體重1.1公斤之牝胎兒一隻。 兔輸卵管液之不足，及山羊發情週期之不定，大為影響移植成績。此二問題有待解決。
- 英文摘要：
Saanen goats were induced to superovulateand were mated at the estrus. They weresubjected to laparotomy 48 to 72 hours afterthe onset of estrus and the embryos wereflushed from the oviducts in a homologous goatserum. The 1- to 4-cell-stage embryos werestored in the rabbit oviduct and were recollected1 to 3 days later for transferring to the uterusof hormonally synchronised Taiwanese goat forfurther development. The stored embryos continued cell cleavagenormally provided the rabbit oviduct was fullof oviduct fluid. Two recollected embryos wereable to continue their growth in the Taiwanesegoat uterus. Of these two, one developed to anormal male kid and the other to a 115-day-oldfemale fetus which died with the recipient goatas it died of acute tympany. Insufficiency of the rabbit ovidct fluid anduncertainty of the length of the estrous cycle ofthe recipient goats limited the success of thisstudy.
- 中文關鍵字：
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- 英文關鍵字：
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