- 作者: 戴達夫
- 作者服務機構: Department of Chemistry, National Dong-Hwa University, Hualien 974, Taiwan, R.O.C.
- 中文摘要: Candida rugosa lipase from Sigma was subjected to an agarose mercurial column, and the three fractions thus collected were further desalted using a Sephadex G-25 column. The purification of these isozymes increases their catalytic activities toward the hydrolysis of triacetin. An improved resolution on the hydrolysis of 2-chloroethyl 2-phenylpropionate was also observed by utilizing one of the isolated isozymes CRL II.
- 英文摘要: --
- 中文關鍵字: Lipase; Agarose mercurial column; Kinetic resolution; 2-Phenylpropionic acid.
- 英文關鍵字: Lipase; Agarose mercurial column; Kinetic resolution; 2-Phenylpropionic acid.