- 作者: 翁啟惠; 陳水田; 王光燦
- 作者服務機構: Institute of Biological Chemistry, Academia Sinica and Department of Chemistry, National Taiwan University, Taipei, Republic of China
- 中文摘要: Preparation of optically pure L-amino acids from the corresponding methyl esters of racemic mixtures by treatment with proteolytic enzyme, bromelain, was investigated. The reaction was carried out at pH 6.0-6.5, 25℃ in a mixture containing 0.1 M substrate, 5 mM β-mercaptoethanol, 0.05M potassium phosphate and 3mg/ml of crude enzyme. The product was isolated by ion exchange chromatography and the purity of product was established by m.p. , tlc, ir spectrum, elemental analysis and optical rotation measurement.
- 英文摘要: --
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